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JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

JoVE | Peer Reviewed Scientific Video Journal - Methods and Prot…

2023年4月30日

SOLVED: A new graduate student is asked to add a nuclear localizationsequence (NLS) to the C-terminus of an ER protein in order tolocalize the protein to the nucleus. Which of the followingstatements would be the best response?A. This won't work since the NLS will be cleaved off of theprotein.B. Great idea, but we should remove the ER signal sequence.C. This won't work since the NLS will not be translated.D. Great idea, we can now see if this protein can function inthe nucleus.

SOLVED: A new graduate student is asked to add a nuclear localizatio…

2021年8月21日

SOLVED: Choose all that apply: The NLS(nuclear localization signal/ sequence) ...- has a consensus basic amino acid residue sequence, KKKRK.-is recognized by a nuclear import receptor protein that is anuclear membrane-bound protein close to the nuclear porecomplexes.-has a consensus acidic amino acid residue sequence, KKKRK.-is a short stretch of amino acyl residues in the proteindestined to the nucleus.-is a GFP tag on the protein being imported into the nucleus-is not the signal sequence for

SOLVED: Choose all that apply: The NLS(nuclear localization signal/ s…

2022年1月23日

Consider a protein that contains an ER signal sequence at its N-terminus and a nuclear localization sequence in its middle. What do you think the fate of this protein would be? Explain your answer. | Numerade

Consider a protein that contains an ER signal sequence at its N-termi…

2020年5月12日

SOLVED: A nuclear localization signal (NLS) is a sequence of amino acidsthat enables a protein to betransported from the cytoplasm to the nucleus (nuclear import).Import of proteins with a nuclear localization signal into thenucleus requires the nucleotideGTP in the nucleus. How/why is GTP, which does not have NLS, ableto enter into the nucleusfrom the cytoplasm? Make sure your answer addresses keycharacteristics of nuclear transportand describes the process of GTP entry into the nucleus.

SOLVED: A nuclear localization signal (NLS) is a sequence of ami…

2021年10月22日

Nuclear

已浏览 195 次10 个月之前

SOLVED: Edit each of the following statements, if required, to make them true: "Because nuclear localization sequences are not cleaved off by proteases following protein import into the nucleus, they can be reused to import nuclear proteins after mitosis, when cytosolic and nuclear proteins have become intermixed. This is in contrast to ER signal sequences, which are cleaved off by a signal peptidase once they reach the lumen of the ER. ER signal sequences cannot therefore be reused to import ER

SOLVED: Edit each of the following statements, if required, to make th…

2021年10月17日

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