Exploring alternative antibody scaffolds: Antibody fragments and ...
2018年12月1日 · In response, the protein engineering community has begun to explore alternative high-binding protein scaffolds as antibody mimics. In this short review I will summarise the generation, modification, and application of emerging antibody fragments and synthetic antibody mimics, with a focus on their use as drug carriers.
Faster, deeper, smaller—the rise of antibody-like scaffolds
2017年7月12日 · Protein scaffolds are much smaller than antibodies—with a molecular weight of anywhere from 2 to 20 kDa, compared with 150 kDa on average for antibodies. That allows them to penetrate into...
Engineering an IgG Scaffold Lacking Effector Function with
2017年2月3日 · IgG2 and IgG4 isotypes have significantly lower binding affinity to Fcγ receptors. Recent evidence suggests that the IgG2 isotype is not completely devoid of effector function, whereas the IgG4 isotype can undergo in vivo Fab arm exchange leading to bispecific antibody and off-target effects.
Protein scaffolds: antibody alternatives for cancer diagnosis and ...
To date, more than 20 types of protein scaffolds have been developed, with the most frequently used being affibody, adnectin, ANTICALIN®, DARPins, and knottin. In this review, we focus on the protein scaffold applications in cancer therapy and diagnosis in the last 5 years, and discuss the pros and cons, and strategies of optimization and design.
Fragment-based computational design of antibodies targeting
2022年11月11日 · Here, we describe a fragment-based method for the combinatorial design of antibody binding loops and their grafting onto antibody scaffolds. We designed and tested six single-domain antibodies targeting different epitopes on three antigens, including the receptor-binding domain of the SARS-CoV-2 spike protein.
Protein scaffolds: antibody alternatives for cancer diagnosis and ...
2022年5月25日 · To date, more than 20 types of protein scaffolds have been developed, with the most frequently used being affibody, adnectin, ANTICALIN®, DARPins, and knottin. In this review, we focus on the protein scaffold applications in cancer therapy and diagnosis in the last 5 years, and discuss the pros and cons, and strategies of optimization and design.
Design Strategy to Create Antibody Mimetics Harbouring …
2020年1月21日 · In this report, we present a method to create such proteins, named fluctuation-regulated affinity proteins (FLAPs). Thirteen graft acceptor (GA) sites that efficiently immobilise the grafted...
Antigen- and scaffold-specific antibody responses to protein
2022年10月18日 · Here we define relationships between anti-scaffold and antigen-specific antibody responses elicited by protein nanoparticle immunogens. We report that dampening anti-scaffold responses by physical masking does not enhance antigen-specific antibody responses.
Nature-inspired design of motif-specific antibody scaffolds
2013年8月18日 · Here we outline a general strategy for producing synthetic, PTM-specific mAbs by engineering a motif-specific 'hot spot' into an antibody scaffold. Inspired by a natural phosphate-binding...
Scaffold Vaccines for Generating Robust and Tunable Antibody Responses
2022年1月8日 · Here, it is found that three scaffold-based vaccines, fabricated from poly (lactide- co -glycolide) (PLG), mesoporous silica rods, and alginate cryogels, induce robust, long-term antibody responses to a model peptide antigen gonadotropin-releasing …
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