miRNA Biogenesis Enzyme Drosha Is Required for Vascular …
2013年4月18日 · The expressions of SMA in Drosha KO and control VSMCs generated using Ade-con and Ade-Cre were examined by immunostaining using SMA antibody; the significant differences were analyzed from four separate experiments by quantifying the fluorescent intensity in the Drosha KO VSMCs compared with controls (*P<0.05).
Expression of the microRNA regulators Drosha, Dicer and …
2015年7月31日 · Drosha, Dicer and Ago2 mRNA and protein expression levels, and their sub-cellular distributions, were assessed using RT-PCR, Western blotting, immunofluorescence and immunohistochemistry, respectively. We found that Drosha, Dicer and Ago2 were expressed in all the cell lines and primary neoplastic and non-neoplastic tissue samples tested.
The mammalian LINC complex component SUN1 regulates muscle …
Here we show that a major muscle specific isoform of the murine LINC complex protein SUN1 is required for efficient muscle regeneration. The nucleoplasmic domain of the isoform specifically binds to and inhibits Drosha, a key component of the …
BRCA1 regulates microRNA biogenesis via the DROSHA …
BRCA1 increased the expressions of both precursor and mature forms of let-7a-1, miR-16-1, miR-145, and miR-34a. In addition, this tumor suppressor was shown to be directly associated with DROSHA and DDX5 of the DROSHA microprocessor complex, and it interacted with Smad3, p53, and DHX9 RNA helicase.
Oncogenic AURKA-enhanced N6-methyladenosine modification …
2020年8月28日 · Here, we demonstrate that DROSHA interacts with β-Catenin to transactivate STC1 in an RNA cleavage-independent manner, contributing to breast cancer stem-like cell (BCSC) properties. DROSHA...
The mammalian LINC complex component SUN1 regulates muscle …
2019年11月5日 · Here we show that a major muscle specific isoform of the murine LINC complex protein SUN1 is required for efficient muscle regeneration. The nucleoplasmic domain of the isoform specifically binds to and inhibits Drosha, a key component of the microprocessor complex required for miRNA synthesis.
Diagnostic and Prognostic Potential of MicroRNA Maturation …
2.1. Immunostaining Pattern of AGO1, AGO2, and Drosha Expression in Bladder Tissue. All three targets appeared with a granular pattern in malignant and non-malignant tissue, while they were also often expressed in endothelial cells (Figure 1 and Figure 2).
SMAD proteins control DROSHA-mediated microRNA maturation
Surprisingly, TGF-β and BMP signalling promotes a rapid increase in expression of mature miR-21 through a post-transcriptional step, promoting the processing of primary transcripts of miR-21 (pri-miR-21) into precursor miR-21 (pre-miR-21) by the DROSHA (also known as …
生命学院王宏伟和生物物理研究所许瑞明课题组合作在《分子细胞 …
2020年3月27日，《分子细胞》杂志在线发表了题为“Drosha识别pri-miRNA的结构基础”（Structural Basis For pri-miRNA Recognition by Drosha）的研究论文，报道了中国科学院生物物理研究所许瑞明研究组和清华大学王宏伟课题组最新合作研究成果，该研究成果解析 …
FLT3-ITD Activates Cytoplasmic Drosha-Dependent Non …
2019年11月13日 · In FLT3-ITD+ blasts, once in the cytoplasm, the unspliced BIC-155 RNA was then processed by cytoplasmic isoforms of DROSHA, as demonstrated using immunostaining, RNA Immunoprecipitation (RIP), and other gain- and loss-of-function experiments.
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