Immunostaining - Wikipedia
In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. [1]
What Is Immunostaining? - Cleveland Clinic
What is immunostaining? Immunostaining is a process pathologists use to diagnose certain diseases, such as infections or cancer. The purpose of immunostaining is to identify a specific protein molecule from a mixture of other proteins in a …
Immunostaining - an overview | ScienceDirect Topics
Immunostaining is a standard technique that employs antibodies to detect and quantify antigen levels. This process uses an antibody targeted against a specific molecule, referred to as the primary antibody, to detect its presence.
Brief guide to immunostaining - ScienceDirect
2025年1月1日 · Immunostaining is an essential biological technique that determines the localization and level of target antigen molecules using antibodies within cells or tissues. Here, we present a brief guide to immunostaining, including the principles, methods, and different types of immunostaining.
Ten Approaches That Improve Immunostaining: A Review of the …
Immunostaining has emerged as one of the most common and valuable techniques that allow the localization of proteins at a quantitative level within cells and tissues using antibodies coupled to enzymes, fluorochromes, or colloidal nanogold particles.
An introduction to Performing Immunofluorescence Staining
Immunofluorescence (IF) is an important immunochemical technique that allows detection and localization of a wide variety of antigens in different types of tissues of various cell preparations. IF allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry, employing various microscopy techniques.
Immunostaining of Skeletal Tissues - PMC - PubMed Central (PMC)
Immunostaining is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, then visualizing the bound antibody using a chromogen (immunohistochemistry or IHC) or fluorescence (immunofluorescence or IF).
Brief guide to immunostaining - PubMed
Immunostaining is an essential biological technique that determines the localization and level of target antigen molecules using antibodies within cells or tissues. Here, we present a brief guide to immunostaining, including the principles, methods, and different types of immunostaining.
Immunostaining: detection of signaling protein location in …
This protocol details the steps involved and appropriate use of both methodologies. Immunostaining is used in cell biology to study differential protein expression, localization and distribution at the tissue, cellular, and subcellular level.
Immunostaining basics: What to know before starting
2023年8月9日 · Immunostaining is a powerful tool that can be used to study protein expression levels, localization, and distribution in tissue or cells. It can also detect more than one target in a sample for co-localization studies.