An introduction to Performing Immunofluorescence Staining
Immunofluorescence (IF) is an important immunochemical technique that allows detection and localization of a wide variety of antigens in different types of tissues of various cell preparations. IF allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry, employing various microscopy techniques.
Immunofluorescence - Wikipedia
Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens.
Immunocytochemistry protocol - Abcam
2022年2月23日 · This guide can be adapted for several applications, from cell surface immunofluorescence staining to whole-cell imaging, both for immunofluorescence double staining or single-label experiments; use this protocol to achieve high-quality, reproducible imaging in …
Multiplexed protein maps link subcellular organization to cellular ...
2018年8月3日 · We report a simple, robust, and nondegrading protocol that achieves 40-plex protein staining in the same biological sample using off-the-shelf antibodies called iterative indirect immunofluorescence imaging (4i).
Immunostaining | Cell Signaling Technology
Immunofluorescence (IF) uses antibodies and fluorescent detection to study the localization, relative expression, and activation states of target proteins in fixed cells or tissues. IF is widely used in diverse fields of study.
免疫染色概述 - Cell Signaling Technology (CST)
免疫荧光法 (IF) 采用抗体和荧光检测来研究固定细胞或组织中靶蛋白的定位、相对表达和激活状态。 IF 广泛应用于不同的研究领域。 IF 可用于解答一些疑问，例如： 哪些细胞或组织表达我的靶蛋白？ 我的靶蛋白表达和/或激活如何随实验干扰而改变？ 靶蛋白定位于细胞/组织的哪个位置？ 在细胞或组织结构环境中，不同靶蛋白之间的空间关系如何？ 免疫荧光法包含了很多随时间逐步形成的相关技术。 所有这些技术都利用抗体对生物靶标的特异性识别和流式细胞分析中对荧光报告 …
Immunofluorescence - ScienceDirect
2017年1月1日 · Immunofluorescence (IF) is a type of immunohistochemistry technique that utilizes fluorophores to visualize various cellular antigens such as proteins. This technique can be utilized to visualize the localization of various cellular components within cells, tissues as well as in 3D culture-derived cellular spherical structures.
Highly multiplexed imaging of single cells using a high …
2015年9月24日 · Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simple and versatile procedure in which four-colour staining...
Spatial multiplexed immunofluorescence analysis reveals …
2024年9月27日 · Recent advancements in spatial, multiplexed immunofluorescence technologies 31 enable simultaneous classification and phenotypic characterization of cellular populations on formalin-fixed,...
Spatiotemporal multiplexed immunofluorescence imaging of …
2022年6月2日 · We demonstrate multiparameter (n ≥ 14), non-disruptive imaging of murine peripheral blood mononuclear and bone marrow cells to profile cellular differentiation. We also show longitudinal...