RNA sequencing refers to techniques used to determine the sequence of RNA molecules. It includes high-throughput shotgun sequencing of cDNA molecules obtained by reverse transcription from RNA ...

So et al. present an optimized protocol for single-nuclei RNA sequencing of adipose tissue in mice, ensuring better RNA quality and nuclei integrity. The authors use this protocol to explore the ...

This repository hosts a comprehensive and modular pipeline for analyzing single-cell RNA sequencing (scRNA-seq) data. The workflow integrates advanced preprocessing, dimensionality reduction, ...

No large RNA structure database exists that correlates sequence with 3D topology. Successful protein-centric methods like AlphaFold remain unavailable for RNA, creating a critical gap in ...

Objectives To integrate published single-cell RNA sequencing (scRNA-seq) data and assess the contribution of synovial fibroblast (SF) subsets to synovial pathotypes and respective clinical ...

Methods We analysed bulk RNA-seq data for enrichment of biological processes, transcription factors (TFs) and deconvolution-based immune cell types from peripheral blood mononuclear cells (PBMCs) in ...