Figure 6: The N-end rule pathway. Subunit selectivity of protein degradation was yet another fundamental feature of the ubiquitin system that was first discovered in the N-end rule pathway.

In eukaryotic cells, a ubiquitin conjugation cascade pathway exists to enzymatically activate ubiquitin, which then becomes covalently attached to substrate proteins that are destined for proteolysis.

E1s and E2s first work generically across the cell to activate and prepare ubiquitin for attachment. E3 ligases then ensure that the tag is added to the right protein; as such, hundreds of specific ...

Subsequent work revealed the structural basis for requisite conformational changes through application of chemical probes that mimicked unstable intermediates in the pathway, showing that the E1 ...

During ubiquitination, a group of enzymes called E3 ubiquitin ligases attach a small ... which investigates ubiquitination pathways in various physiological contexts, developed a simple, cost ...

A new review published in Genes & Diseases explores the pivotal role of the ubiquitin-proteasome system (UPS) in regulating the tumor microenvironment (TME) and driving cancer progression. The study ...