Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

Neurons, neural stem cells, and other slowly dividing cells are notoriously difficult to transfect. With CRISPR-based gene editing gaining momentum, transfection is an essential technique in the ...

图 1. Attractene效率更高. DNA (pGFP which expresses green fluorescent protein) was transfected into the cell types indicated using Attractene Reagent. Transfection were also performed with A Reagent F or B ...

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Electroporation uses short electric pulses to create transient pores in a cell’s membrane, allowing researchers to deliver intracellular ... to discover an approach using the Neon NxT system that ...

CellFE, a leader in non-viral gene editing technology, today announced the launch of its highly anticipated CellFE T-Rest ...

The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...